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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">detstom</journal-id><journal-title-group><journal-title xml:lang="ru">Стоматология детского возраста и профилактика</journal-title><trans-title-group xml:lang="en"><trans-title>Pediatric dentistry and dental prophylaxis</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">1683-3031</issn><issn pub-type="epub">1726-7218</issn><publisher><publisher-name>RPA</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.33925/1683-3031-2024-815</article-id><article-id custom-type="elpub" pub-id-type="custom">detstom-815</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ОРИГИНАЛЬНАЯ СТАТЬЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>ORIGINAL ARTICLE</subject></subj-group></article-categories><title-group><article-title>Изучение эффективности применения  ультразвукового и механического способов  для определения степени деминерализации  дентина постоянных зубов у детей in vitro</article-title><trans-title-group xml:lang="en"><trans-title>Evaluation of ultrasonic and mechanical methods for assessing dentin demineralization in children's permanent teeth in vitro</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-6740-3363</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Седойкин</surname><given-names>А. Г.</given-names></name><name name-style="western" xml:lang="en"><surname>Sedoykin</surname><given-names>A. G.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Седойкин Алексей Геннадьевич, кандидат медицинских наук, доцент кафедры детской стоматологии член-корреспондент РАН</p><p>Москва</p></bio><bio xml:lang="en"><p>Alexey G. Sedoykin, DMD, PhD, Associate Professor, Department of the Pediatric Dentistry</p><p>Moscow </p></bio><email xlink:type="simple">alexdokt_01@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-4219-3547</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ермольев</surname><given-names>С. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Ermoljev</surname><given-names>S. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ермольев Сергей Николаевич, доктор медицинских наук, профессор кафедры терапевтической стоматологии и пародонтологии</p><p>Москва</p></bio><bio xml:lang="en"><p>Sergey N. Ermoljev, DMD, PhD, DSc, Professor, Department of Restorative Dentistry and Periodontology</p><p>Moscow </p></bio><email xlink:type="simple">ermoljev_s@hotmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-2095-9473</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Кисельникова</surname><given-names>Л. П.</given-names></name><name name-style="western" xml:lang="en"><surname>Kiselnikova</surname><given-names>L. P.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Кисельникова Лариса Петровна, заслуженный врач РФ, доктор медицинских наук, профессор, заведующая кафедрой детской стоматологии</p><p>Москва</p></bio><bio xml:lang="en"><p>Larisa P. Kiselnikova, DMD, PhD, DSc, Professor, Head of the Department of Pediatric Dentistry</p><p>Moscow </p></bio><email xlink:type="simple">lpkiselnikova@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-0522-2860</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Фокина</surname><given-names>А. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Fokina</surname><given-names>A. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Фокина Александра Алексеевна, ассистент кафедры ортодонтии, врач-ортодонт отделения ортодонтии</p><p>Москва</p></bio><bio xml:lang="en"><p>Alexandra A. Fokina, DMD, Assistant Professor, Department and Clinic of Orthodontics</p><p>Moscow </p></bio><email xlink:type="simple">fokina.aleksandra@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-6073-9814</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Гонибова</surname><given-names>А. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Gonibova</surname><given-names>A. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Гонибова Алена Альбертовна, кандидат медицинских наук, ассистент кафедры ортопедической стоматологии</p><p>Москва</p></bio><bio xml:lang="en"><p>Alena A. Gonibova, DMD, PhD, Assistant Professor, Department of Prosthodontics</p><p>Moscow </p></bio><email xlink:type="simple">6261854@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Российский университет медицины</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Russian University of Medicine</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2024</year></pub-date><pub-date pub-type="epub"><day>24</day><month>10</month><year>2024</year></pub-date><volume>24</volume><issue>3</issue><fpage>307</fpage><lpage>312</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Седойкин А.Г., Ермольев С.Н., Кисельникова Л.П., Фокина А.А., Гонибова А.А., 2024</copyright-statement><copyright-year>2024</copyright-year><copyright-holder xml:lang="ru">Седойкин А.Г., Ермольев С.Н., Кисельникова Л.П., Фокина А.А., Гонибова А.А.</copyright-holder><copyright-holder xml:lang="en">Sedoykin A.G., Ermoljev S.N., Kiselnikova L.P., Fokina A.A., Gonibova A.A.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.detstom.ru/jour/article/view/815">https://www.detstom.ru/jour/article/view/815</self-uri><abstract><sec><title>Актуальность</title><p>Актуальность. С появлением усовершенствованных способов функциональной диагностики у стоматолога возникают новые возможности выявления очагов кариозного поражения, однако диагностическая чувствительность данных способов требует изучения.</p></sec><sec><title>Цель исследования</title><p>Цель исследования: изучить состояние интактного и деминерализованного дентина постоянных зубов у детей ультразвуковым и механическим способами.</p></sec><sec><title>Материалы и методы</title><p>Материалы и методы. Модели «разделенный шлиф» изготавливались из зубов группы премоляров, удаленных у детей по ортодонтическим показаниям. После 20-дневной экспозиции в деминерализирующем буферном растворе с рН = 4,5, интактная и деминерализованная часть дентина модели подвергались испытаниям ультразвуковым и механическим способами.</p></sec><sec><title>Результаты</title><p>Результаты. Выявлены статистически значимые различия средних значений скорости ультразвукового сигнала – «С» в двух независимых группах образцов с интактным (гр. 1) и деминерализованным дентином (гр. 2) согласно расчетам U-критерия Манна – Уитни (при p = 0,01). В образцах (гр. 2) выявлено снижение показателя среднего значения скорости ультразвукового сигнала «С» на 27%, чем в образцах (гр. 1). Получены статистически значимые различия средних значений предельного разрушающего напряжения – «σ» в двух независимых группах образцов с интактным (гр. 3) и деминерализованным дентином (гр. 4) согласно расчетам U-критерия Манна – Уитни (при p = 0,01). В образцах (гр. 4) показатель среднего значения предельного разрушающего напряжения «σ» был на 29,5% меньше, чем в образцах (гр. 3).</p></sec><sec><title>Заключение</title><p>Заключение. В процессе деминерализации происходит изменение ультразвуковых и механических свойств дентина. Статистический анализ результатов лабораторных испытаний модели «разделенный шлиф» подтверждает эффективность и диагностическую чувствительность способов, использованных для оценки степени деминерализации дентина.</p></sec></abstract><trans-abstract xml:lang="en"><sec><title>Relevance</title><p>Relevance. With the advent of advanced methods for functional diagnostics, dentists have gained new opportunities for detecting carious lesions. However, the diagnostic sensitivity of these methods requires further investigation</p></sec><sec><title>Purpose</title><p>Purpose. To evaluate the condition of intact and demineralized dentin in children's permanent teeth using both ultrasonic and mechanical methods.</p></sec><sec><title>Materials and methods</title><p>Materials and methods. Divided cross-section" models were created from premolars extracted from children for orthodontic purposes. After a 20-day exposure to a demineralizing buffer solution with a pH of 4.5, both the intact and demineralized dentin cross-sections were tested using ultrasonic and mechanical methods.</p></sec><sec><title>Results</title><p>Results. Statistically significant differences were observed in the mean ultrasonic signal speed C between two independent sample groups: intact dentin (Group 1) and demineralized dentin (Group 2), based on Mann-Whitney U-test calculations (p = 0.01). In Group 2, the mean value of C was 27% lower compared to Group 1. Additionally, significant differences were found in the mean ultimate strength σ between two independent groups: intact dentin (Group 3) and demineralized dentin (Group 4), with Group 4 showing a 29.5% reduction in the mean σ value, according to Mann-Whitney U-test calculations (p = 0.01).</p></sec><sec><title>Conclusion</title><p>Conclusion. Demineralization leads to significant changes in both the ultrasonic and mechanical properties of dentin. Statistical analysis of the laboratory test results using the "divided cross-section" model supports the effectiveness and diagnostic sensitivity of the methods applied for evaluating the extent of dentin demineralization. </p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>искусственная деминерализация дентина</kwd><kwd>механические свойства дентина</kwd><kwd>ультразвуковые свойства дентина</kwd></kwd-group><kwd-group xml:lang="en"><kwd>artificial dentin demineralization</kwd><kwd>mechanical properties of dentin</kwd><kwd>ultrasonic properties of dentin</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Wang Y, Yao X. Morphological/chemical imaging of demineralized dentin layer in its natural, wet state. Dent Mater. 2010;26(5):433-42. doi: 10.1016/j.dental.2010.01.002</mixed-citation><mixed-citation xml:lang="en">Wang Y, Yao X. Morphological/chemical imaging of demineralized dentin layer in its natural, wet state. Dent Mater. 2010;26(5):433-42. doi: 10.1016/j.dental.2010.01.002</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Saeki K, Chien YC, Nonomura G, Chin AF, Habelitz S, Gower LB, et al. Recovery after PILP remineralization of dentin lesions created with two cariogenic acids. Arch Oral Biol. 2017;82:194-202. doi: 10.1016/j.archoralbio.2017.06.006</mixed-citation><mixed-citation xml:lang="en">Saeki K, Chien YC, Nonomura G, Chin AF, Habelitz S, Gower LB, et al. Recovery after PILP remineralization of dentin lesions created with two cariogenic acids. Arch Oral Biol. 2017;82:194-202. doi: 10.1016/j.archoralbio.2017.06.006</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Xu C, Wang Y. Cross-linked demineralized dentin maintains its mechanical stability when challenged by bacterial collagenase. J Biomed Mater Res B Appl Biomater. 2011;96(2):242-8. doi: 10.1002/jbm.b.31759</mixed-citation><mixed-citation xml:lang="en">Xu C, Wang Y. Cross-linked demineralized dentin maintains its mechanical stability when challenged by bacterial collagenase. J Biomed Mater Res B Appl Biomater. 2011;96(2):242-8. doi: 10.1002/jbm.b.31759</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Nishitani Y, Yoshiyama M, Tay FR, Wadgaonkar B, Waller J, Agee K, et al. Tensile strength of mineralized/ demineralized human normal and carious dentin. J Dent Res. 2005;84(11):1075-8. doi: 10.1177/154405910508401121</mixed-citation><mixed-citation xml:lang="en">Nishitani Y, Yoshiyama M, Tay FR, Wadgaonkar B, Waller J, Agee K, et al. Tensile strength of mineralized/ demineralized human normal and carious dentin. J Dent Res. 2005;84(11):1075-8. doi: 10.1177/154405910508401121</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Y Chien YC, Burwell AK, Saeki K, Fernandez-Martinez A, Pugach MK, Nonomura G, et al. Distinct decalcification process of dentin by different cariogenic organic acids: Kinetics, ultrastructure and mechanical properties. Arch Oral Biol. 2016;63:93–105. doi: 10.1016/j.archoralbio.2015.10.001.</mixed-citation><mixed-citation xml:lang="en">Y Chien YC, Burwell AK, Saeki K, Fernandez-Martinez A, Pugach MK, Nonomura G, et al. Distinct decalcification process of dentin by different cariogenic organic acids: Kinetics, ultrastructure and mechanical properties. Arch Oral Biol. 2016;63:93–105. doi: 10.1016/j.archoralbio.2015.10.001.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Panfilov P, Zaytsev D, Antonova OV, Alpatova V, Kiselnikova LP. The Difference of Structural State and Deformation Behavior between Teenage and Mature Human Dentin. International Journal of Biomaterials. 2016;2016:6073051. doi: 10.1155/2016/6073051</mixed-citation><mixed-citation xml:lang="en">Panfilov P, Zaytsev D, Antonova OV, Alpatova V, Kiselnikova LP. The Difference of Structural State and Deformation Behavior between Teenage and Mature Human Dentin. International Journal of Biomaterials. 2016;2016:6073051. doi: 10.1155/2016/6073051</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
